This post does NOT constitute medical advice. It is a synthesis of lab-driven reasoning based on a Foundation Zoomer and related gut findings (elevated Anti-LPS, zonulin signals, Gut Score 71, Liver sub-score 65, HOMA-IR 2.7). Work with a knowledgeable clinician before making changes.

TL;DR

Morning – Empty Stomach
L-Glutamine 5 g + Zinc Carnosine 75 mg + Taurine 1 g + NAC 1 g + Glycine 5 g + Apolactoferrin 250 mg + Florastor + Sunfiber 6 g + Bifidobacterium lactis + Boron 3–6 mg

Morning – With Food
Fish Oil (high EPA/DHA) + Siliphos 300 mg + TriButyrin 300 mg + Quercetin Phytosome 250–500 mg (+ optional UC-II 40 mg and/or bioavailable curcumin)

Evening – Before Bed
Glycine 10 g + Magnesium L-Threonate + Taurine 1 g + NAC 1 g + Fish Oil + Apigenin 50 mg + L-Theanine 200 mg + Mushroom complex (optional) + TA-65 (optional)

Core goal: Repair intestinal barrier, lower endotoxin load, support liver, improve insulin sensitivity, and create a cleaner cellular environment via periodic 50–65 hour fasts.

Introduction

The protocol below was built around the most actionable findings from the July 2026 Foundation Zoomer:

• Gut Score 71 (Moderate) with clear barrier stress signals (Anti-LPS, zonulin)
• Liver sub-score 65 and history of elevated GGT/ALT
• HOMA-IR 2.7 (insulin resistance)
• Secondary immune activation (immature granulocytes, basophils)
• Cardiovascular score 73

The stacks prioritize evidence-aligned tools that directly address intestinal permeability, endotoxin translocation, short-chain fatty acid support, liver membrane and antioxidant status, and systemic inflammation. Periodic 50–65 hour water fasts are used as a complementary stimulus for autophagy and mitophagy.

Morning Stack – Empty Stomach / Early

Supplement	Dose	Primary Purpose	Why It Matters
L-Glutamine	5 g	Enterocyte fuel & tight junction support	Primary metabolic fuel for intestinal lining cells; foundational for barrier repair
Zinc Carnosine	75 mg complex	Mucosal stabilization	Directly supports tight junctions and reduces permeability; one of the strongest evidence-based barrier agents
Taurine	1 g	Bile support + mild calming	Supports bile flow and liver function; pairs well with fasting and refeeds
NAC	1 g	Glutathione & liver support	Boosts glutathione; helps with oxidative stress and elevated liver enzymes
Glycine	5 g	Barrier + glutathione + calming	Supports collagen/barrier structures and glutathione synthesis
Apolactoferrin	250 mg	LPS binding	Helps bind and reduce bacterial endotoxin load
Florastor (S. boulardii)	250 mg	Barrier & pathogen resistance	Yeast probiotic with data for supporting intestinal integrity
Sunfiber (PHGG) + B. lactis	6 g + 8 billion CFU	SCFA production & Bifido support	Raises butyrate and other SCFAs; feeds beneficial bacteria
Boron	3–6 mg	Mild anti-inflammatory + joint support	May help with inflammation and has some osteoarthritis data

Reasoning: These are the highest-leverage tools for the Gut Score of 71 and elevated permeability/LPS signals. They work best on an empty or near-empty stomach so they can interact directly with the intestinal lining.

Morning Stack – With Food

Supplement	Dose	Primary Purpose	Why It Matters
Fish Oil	~1.5–2 g combined EPA+DHA	Anti-inflammatory & lipid support	Addresses systemic inflammation and cardiovascular score; supports resolution of inflammatory signaling
Siliphos (Milk Thistle Phytosome)	300 mg	Liver membrane & enzyme support	Targets the weak Liver sub-score (65) and elevated enzymes
TriButyrin	300 mg	Direct butyrate delivery	Fuels colonocytes and supports barrier integrity from the colonic side
Quercetin Phytosome	250–500 mg	Tight junction & mast cell support	Complements glutamine and zinc carnosine; reduces LPS-driven inflammation
UC-II (optional)	40 mg	Joint / oral tolerance	Evidence-based for osteoarthritis symptoms
Bioavailable Curcumin (optional – Thorne Meriva preferred)	500–1000 mg Meriva or equivalent	Anti-inflammatory	Additional joint and systemic inflammation support

Reasoning: These ingredients absorb better with food (especially fat) and provide complementary anti-inflammatory, liver, and butyrate support without competing with the empty-stomach barrier agents.

Evening Stack

Supplement	Dose	Primary Purpose	Why It Matters
Glycine	10 g	Sleep quality + continued barrier support	Improves sleep architecture and supplies ongoing glycine for repair
Magnesium L-Threonate	~725 mg (or as labeled)	Brain magnesium & sleep	Supports relaxation, sleep quality, and recovery
Taurine	1 g	Continued liver/bile + calming	Maintains support overnight
NAC	1 g	Overnight glutathione & liver support	Keeps antioxidant capacity elevated
Fish Oil	Split dose	Steady anti-inflammatory coverage	Maintains omega-3 levels
Apigenin	50 mg	Sleep pressure & fewer awakenings	Gentle GABA modulation
L-Theanine	200 mg	Reduced mental arousal	Helps with sleep onset and continuity
Mushroom complex (optional)	~1 g	Immune modulation & calming	Reishi in particular supports evening wind-down
TA-65 (optional)	As directed	Telomerase support	Longevity-oriented; not core to the lab findings

Reasoning: Evening dosing emphasizes recovery, sleep quality (which itself supports barrier repair and metabolic health), and continued low-level support for liver and glutathione status.

How the Stacks Map to the Labs

• Gut Score 71 / Anti-LPS / Zonulin → Glutamine, Zinc Carnosine, TriButyrin, Sunfiber, Apolactoferrin, Quercetin, Florastor
• Liver sub-score 65 → Siliphos, NAC, Taurine, Glycine
• HOMA-IR 2.7 → Omega-3s, reduced inflammatory load, periodic fasting, better sleep
• Systemic inflammation & immune activation → Fish oil, Quercetin, Apolactoferrin, lower LPS translocation
• Sleep & recovery → High-dose Glycine, Magnesium L-Threonate, Apigenin, L-Theanine, Taurine

Fasting Integration (50–65 Hours)

Three well-spaced 50–65 hour water fasts over 8–12 weeks are used to drive autophagy and mitophagy. During the fast, prioritize electrolytes, magnesium, taurine, and NAC. Pause the higher-amino-acid and caloric items (glutamine, high-dose glycine, Sunfiber, fish oil, Siliphos, etc.) until the gentle refeed.

Mitophagy (selective clearance of damaged mitochondria) is particularly relevant to gut health because intestinal epithelial cells require healthy mitochondria to maintain tight junctions and control local ROS and inflammation.

Expected Timeline

Weeks 1–4: Focus on consistency and tolerance. Barrier-support nutrients begin working.
Weeks 4–8: First and second 50–65 hour fasts. Progressive reduction in inflammatory and permeability signals expected.
Weeks 8–12: Third fast + retesting of key markers (Gut Score / permeability markers, liver enzymes, HOMA-IR).

Final Notes

This protocol is deliberately layered: daily high-leverage barrier and liver support, evening recovery and sleep optimization, and periodic deep autophagy/mitophagy stimuli. The largest expected improvements are in gut barrier markers, liver enzymes, and insulin sensitivity.

Reassess with labs at 8–12 weeks and adjust. The goal is measurable progress on the weakest scores, not indefinite high-intensity supplementation.